Structure-Guided design of Cas12a variants improves detection of nucleic acids.

• Tác giả: Tianle Li, Xiaohan Tong, Hao Yin, Kun Zhang, Ying Zhang, Dongming Zhao
• Ngôn ngữ: eng
• Ký hiệu phân loại: 794.10285 Chess
• Thông tin xuất bản: Netherlands : Cell insight , 2025
• Bộ sưu tập: NCBI
• ID: 684062

Tóm tắt

CRISPR-Cas12a holds promising potential for pathogen detection. However, its performance is not optimal when combined with isothermal amplification. Hence, we engineered a mutant of LbCas12a (K595A) with reduced