BACKGROUND: Intraoperative determination of resection margin and adequate residual liver parenchyma are the key points of hepatectomy for the treatment of liver tumors. Intraoperative ultrasound and indocyanine green fluorescence navigation are the most commonly used methods at present, but the technical barriers limit their promotion. AIM: To evaluate the value of the three-dimensional location approach with silk thread (3D-LAST) in precise resection of liver tumors. METHODS: From September 2020 to January 2022, 8 patients with liver tumors including hepatocellular carcinoma, intrahepatic cholangiocarcinoma, hilar cholangiocarcinoma, and gastric cancer liver metastasis were included in this study. All patients underwent 3D-LAST in precise resection of liver tumors. RESULTS: All patients (8/8, 100%) underwent the operation successfully without any complications. During the mean follow-up of 8.7 months, all patients survived without tumor recurrence. CONCLUSION: In conclusion, the 3D-LAST is a safe and effective new method for liver intraoperative navigation, which is practical and easy to promote.