Human tendon stem/progenitor cell-derived extracellular vesicle production promoted by dynamic culture.

• Tác giả: Maria Camilla Ciardulli, Marta Clerici, Tina P Dale, Giovanna Della Porta, Nicholas R Forsyth, Emanuele Giordano, Erwin Pavel Lamparelli, Joseph Lovecchio, Nicola Maffulli
• Ngôn ngữ: eng
• Ký hiệu phân loại: 128.5 Human death
• Thông tin xuất bản: England : Artificial cells, nanomedicine, and biotechnology , 2025
• Bộ sưu tập: NCBI
• ID: 684663
• Chủ đề: 1. Human; 2. Tendon; 3. Stem

Tóm tắt

Tendon injuries significantly impact quality of life, prompting the exploration of innovative solutions beyond conventional surgery. Extracellular Vesicles (EVs) have emerged as a promising strategy to enhance tendon regeneration. In this study, human Tendon Stem/Progenitor Cells (TSPCs) were isolated from surgical biopsies and cultured in a Growth-Differentiation Factor-5-supplemented medium to promote tenogenic differentiation under static and dynamic conditions using a custom-made perfusion bioreactor. Once at 80% confluence, cells were transitioned to a serum-free medium for conditioned media collection. Ultracentrifugation revealed the presence of vesicles with a 10