This study evaluated the strength, hardness, and color stability of 3D-printed denture base resins and compared the outcome with conventional heat-cured denture base resins after aging by thermocycling. A total of 72 specimens from conventional and 3D-printed materials were fabricated in different shapes and dimensions based on the mechanical and color tests performed. The specimens were divided into five groups: flexural, tensile, and compressive strengths (n = 20), hardness, and color stability (n = 6). In all these groups, half of the specimens were stored in a distilled water bath at 37 °C for 24 h, and the remaining half of the specimens were subjected to aging by thermocycling. The 3D-printed specimens demonstrated the highest means of tensile strength (32.20 ± 3.8 MPa), compressive strength (106.31 ± 4.07 MPa), and Vickers hardness number (24.51 ± 0.36), and the lowest means of flexural strength (54.29 ± 13.17 MPa) and color difference (ΔE = 2.18 ± 1.09). Conventional heat-cured specimens demonstrated the highest means of flexural strength (59.96 ± 8.39 MPa) and color difference (ΔE = 4.74 ± 2.37) and the lowest means of tensile strength (32.17 ± 9.06 MPa), compressive strength (46.05 ± 4.98 MPa), and Vickers hardness number (10.42 ± 1.05). Aging significantly reduced the flexural strength (-27%), tensile strength (-44%), and hardness (-7%) of 3D-printed resins in contrast to the conventional resin's compressive strength (-15%) and color stability (