Viruses are significant human pathogens responsible for pandemic outbreaks and seasonal epidemics. Viral infectious diseases impose a devastating global burden and have a profound impact on public health systems. During viral infections, alternative splicing (AS) plays a crucial role in regulating immune responses, altering the host's cellular environment, expanding viral genetic material, and facilitating viral replication. As research on AS in viral infections expands, it is crucial to consolidate data on virus-related splicing changes to improve our understanding of these viruses and associated diseases. To address this need, we created ASVirus (https://bddg.hznu.edu.cn/asvirus/), a comprehensive database of virus-associated AS events and their regulatory factors. ASVirus uniquely combines high-confidence, experimentally validated splicing data and investigates upstream regulatory mechanisms through a gene-splicing factor interaction network. Its user-friendly web interface offers detailed information into AS events from various viral families and the resulting mis-splicing in host genes, aiding the exploration of novel viral infection mechanisms and the identification of critical therapeutic targets for viral diseases.