In this study, perilla seed oil body protein (PO), hempseed oil body protein (HO), and cucumber seed oil body protein (CO) were utilized to prepare artificial oil bodies (AOBs), and the impact of protein type on their physicochemical properties was investigated. Compared to HO and CO, PO exhibited a lower zeta potential value of -48.27 mV, a higher surface hydrophobicity of 21,122.67, and a smaller particle size of 105.71 nm. Its emulsifying activity index (EAI) and emulsion stability index (ESI) were 83.06 m