Artifactual elevations of 11β-hydroxyandrostenedione and 11-ketoandrostenedione in mass spectrometry assays.

• Tác giả: Richard J Auchus, Chaelin Lee, Patrick O'Day, David G Stouffer, Adina F Turcu
• Ngôn ngữ: eng
• Ký hiệu phân loại: 942.0174 *England and Wales
• Thông tin xuất bản: England : The Journal of steroid biochemistry and molecular biology , 2025
• Bộ sưu tập: NCBI
• ID: 685626

Tóm tắt

Comprehensive steroid profiling by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) can be achieved using small biospecimen samples. LC-MS/MS assays offer superior accuracy to immunoassays, but they also introduce layers of complexity and opportunities for errors. Validation and harmonization studies are essential to ensure reliable results, as these assays are being increasingly incorporated in clinical laboratories. We developed an LC-MS/MS assay that simultaneously quantifies 18 Δ