A high affinity Sybody blocks Cofilin-1 binding to F-actin in vitro and in cancer cells.

• Tác giả: Marcus Deckers, Jan Faix, Soo-Ji Han, Jing Li, Themistoklis Paraschiakos, Vivian Pogenberg, Jonas Scholz, Sabine Windhorst
• Ngôn ngữ: eng
• Ký hiệu phân loại: 658.32259 Personnel management (Human resource management)
• Thông tin xuất bản: England : Biochemical pharmacology , 2025
• Bộ sưu tập: NCBI
• ID: 685646

Tóm tắt

Upregulation of the actin-severing protein Cofilin-1 is implicated in enhancing malignancy of various cancer types by promoting actin turnover and increasing cellular motility. Despite the importance of targeting Cofilin-1, currently there is a lack of inhibitors specifically targeting its actin-severing activity. To address this issue, we generated synthetic anti-Cofilin-1 nanobodies (Sybodies) that interfere with human Cofilin-1 binding to filamentous actin. We identified four high affinity Sybodies against human Cofilin-1 with dissociation constants (K