Recombination Analysis of Geminiviruses Using Recombination Detection Program (RDP).

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Tác giả: Alvin Crespo-Bellido, Siobain Duffy, Darren Patrick Martin

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: United States : Methods in molecular biology (Clifton, N.J.) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 685916

Geminiviruses are recombination-prone, and characterizing this evolutionary process within their genomes is a frequent goal of researchers. RDP is a stand-alone Windows program combining many algorithms that detect and characterize recombination. It has been widely used by the geminivirus community (and beyond). Here we describe the use of RDP4 and RDP5 for analysis of geminiviral nucleotide sequences including: (i) obtaining a reasonable dataset for analysis, (ii) making a credible multiple sequence alignment and (iii) analyzing an alignment with RDP on that alignment. RDP to both characterize recombination events and to produce statistically recombination-free datasets for other molecular evolution analyses.
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