Anthracophyllum hirsutum and Stereum hirsutum produce manganese peroxidase (MnP) and laccase to break down lignin, a potential biological pretreatment for lignocellulosic biomass. This work aimed to evaluate the effect of copper (Cu) and manganese (Mn) added to olive mill solid waste (OMSW) inoculated with A. discolor and S. hirsutum on ligninolytic enzyme activity, lignin degradation, and phenolic compound removal. Different optimal metal dosages were determined for each fungal strain. For S. hirsutum, the addition of 6.1 mg Cu kg⁻