This study focused on the synthesis of plant-mediated copper-oxide nanoparticles (OsCuONPs) via the sol-gel technique and the fabrication of OsCuONP-infused PVA composite films (POsCuONPs) utilizing the solvent casting method for wound-healing applications. The prepared OsCuONPs and nanocomposite films were characterized using UV-visible spectra, FTIR, SEM, XRD, TGA, water contact-angle (WCA) measurements, and a Universal testing machine (UTM) for mechanical property measurements. The UV and FTIR tests showed that OsCuONPs were formed and were present in the PVA composite film. Moreover, the mechanical study confirmed that there is an increase in the tensile strength (