Water pollution by metals and metalloids promotes toxic effects to aquatic biota especially in mining regions. Environmental legislation applied to protect aquatic life from the toxicity of metals relies on the definition of protective values (PVs) for each compound. Among methods used to define PVs, Species Sensitivity Distribution (SSD) curves enable the derivation of the Predicted No Effect concentration (PNEC). In this context, this is one of the first studies to propose the construction of acute and chronic split SSD curves built separately for three groups of freshwater organisms (algae, invertebrates and fish) to derive PNEC values for the 14 metals most commonly observed in iron ore mining tailings. Data used to construct split SSD curves were derived from the USEPA ECOTOX knowledgebase and EnviroTox databases and segregated according to the freshwater organism group and as "acute" or "chronic" toxicity. Then, split SSD curves were built using a minimum of nine species for each group to determine the hazardous concentration to 5% of species (HC