Water-borne infections are considered as one of the major risky concerns regarding the sanitary state of water bodies dedicated to drinking water supply. Therefore, the employment of environmentally benign materials in water/wastewater treatment is an indispensable aspect to solve the water crisis problem in an eco-friendly and economic manner. This study describes the synthesis, characterization, and disinfection potency of different formulas of gelatin-based Gum Arabic composites, for the first time. SEM, XRD, FTIR, ζ-potential, and swelling tests were used to assess their physicochemical properties, which revealed the enhanced compatibility and miscibility with increasing Gum Arabic concentration. The formula of GEL/50%GA showed more homogenously distributed pores as visualized by SEM with noticeable shifts in the characteristic FTIR-band and more negatively charged surface, reflecting the considerable stability as indicated by ζ-potential. Besides, it also had superior hydrophilic and swellability levels. Interestingly, the results of antimicrobial activity showed the susceptibility of broad-spectrum microbes against examined composites, especially with elevating the concentration of Gum Arabic incorporated in the composite. As a natural alternative disinfectant, the as-prepared composites (3 and 10% W/V) were evaluated in the disinfection of real wastewater samples. The results revealed that GEL/50%GA (10% W/V) exhibited a noticeable reduction in total plate count by 45.62 ± 1.48% and 37.48 ± 1.63% and in coliforms by 58.43 ± 2.07% and 40.88 ± 2.24% for municipal and industrial effluents, respectively. However, the microbial metabolic activity via MTT assay was diminished by more than 50% in both effluents
denoting the efficient inhibiting capability of GEL supplemented with GA films in restricting microbial viability even in unculturable microbes. Overall, the antagonistic activity of examined composites offers promising insights for recruitment in different disciplines such as anti-biofouling membranes, food coating, dietary supplements, wound healing, and drug delivery.