This study investigated the effects of up to 45% inclusion of whole pulse ingredients in grain-free (GF) diets on the excretion of bile acids (BA) and other fecal metabolites in healthy large-breed dogs. Twenty-eight adult Siberian Huskies were fed one of four experimental diets formulated to meet the same macronutrient profiles for 20 weeks: 1) grain-inclusive diet with 45% corn (Ctl), 2) GF diet with 15% pulses (Pulse15), 3) GF diet with 30% pulses (Pulse30), 4) GF diet with 45% pulses (Pulse45). All diets included chicken meal and pea starch. Fecal samples were collected on week 2 and 19. Bile acids were analyzed using ultra-performance liquid chromatography-MRM/MS technology, while fecal metabolites were analyzed using Agilent HP1000 high-performance liquid chromatography. Bile acid and fecal metabolite data were analyzed using the PROC GLIMMIX procedure in SAS studios (SAS version 9.4, SAS Inst., Inc., Cary, NC). All means were separated using the Tukey-Kramer adjustment (significant when P<
0.05). After 20 weeks of feeding, concentrations of lithocholic acid were greater in Pulse15 and Pulse30 than Ctl (P=0.001), but all were similar to Pulse45. Concentrations of deoxycholic (P=0.054), lithocholic (P=0.001), total secondary (P=0.022), and total BA (P=0.045) tended to be linearly associated with dietary pulse inclusion. Dogs consuming Pulse30 had greater fecal propionic acid concentrations than Ctl (P=0.017), but both were similar to Pulse15 and Pulse45. Total branched-chain fatty acids (P=0.001) and iso-butyric acid (P<
0.0001) were greater in Ctl than in all pulse groups. Inversely, arabinose concentrations were greater in all pulse groups compared to Ctl (P=0.001). In summary, diets with up to 45% inclusion of whole pulse ingredients do not increase total BA excretion but may contribute to greater SCFA production.