BACKGROUND: The rising prevalence of type II diabetes mellitus (T2DM) has increased the risk of hepatic dysfunction, necessitating effective therapeutic interventions. Natural products, particularly OBJECTIVES: This study investigates the hepatoprotective effects of BA, WS, and their polyherbal combination in streptozotocin-nicotinamide-induced T2DM in Wistar rats. METHODS: Seventy-eight adult albino Wistar rats were divided into 13 groups, including normal control, disease control, and treatment groups, which received BA, WS, and a polyherbal combination (PHC) at varying doses (250, 500, and 1,000 mg/kg). Metformin and glimepiride served as standard treatments. Liver function was assessed through serum glutamic pyruvic transaminase (SGPT) and bilirubin levels, and histopathological analysis was performed. RESULTS: Treatment with BA, WS, and PHC significantly reduced SGPT and bilirubin levels compared to the disease control (p <
0.001). The PHC demonstrated superior efficacy, with higher doses (1,000 mg/kg) exhibiting results comparable to standard drugs. Histopathological analysis revealed reduced hepatocellular damage in treated groups, indicating preserved hepatic architecture. CONCLUSION: BA and WS, individually and in combination, significantly attenuated liver dysfunction in diabetic rats. The synergistic effect of PHC presents a promising natural therapeutic approach for managing diabetes-induced liver damage.