Development and evaluation of BDNF-loaded PCL/PVA two-layer nerve guidance conduit with enhanced biomechanical and biological properties for peripheral nerve regeneration.

• Tác giả: Massoumeh Jabbari Fakhr, Mohsen Eslami Farsani, Leyla Fath-Bayati, Fatemeh Kavakebian, Alireza Rezapour, Mehdi Sadrjahani, Reihaneh Seyedebrahimi
• Ngôn ngữ: eng
• Thông tin xuất bản: England : Heliyon , 2025
• Bộ sưu tập: NCBI
• ID: 687230

Tóm tắt

The repair of damaged peripheral nerves and the following restoration of functionality remain significant therapeutic challenges. Hollow nerve conduits currently available do not align with the ideal human model. Successfully mending nerve gaps requires incorporating biomimetic and functional features into neural conduit design. In this research, a new two-layer conduit that combines topographic support and controlled growth factor release was developed. We used a two-layered framework to amplify the mechanical reinforcement and reduce the risk of tissue collapse post-grafting. The hollow nerve conduits were fabricated through three-dimensional printing, employing Polycaprolactone (PCL) and a slowly biodegradable nanofiber for the intraluminal brain-derived neurotrophic factors (BDNF)-loaded polyvinyl alcohol (PVA)/PCL core-shell. The contact angle was indicated to show the hydrophilicity properties and degradation rate for biocompatibility. The scanning electron microscope