Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer.

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Tác giả: Xiaohui Du, Qingzhe Jia, Yanan Jiao, Bin Lan, Zeqiu Lao, Songyan Li, Jing Lou, Zhaofu Ma, Wenbin Shen, Yujia Wang, Mingjin Zhang, Guijun Zou

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: United States : Research (Washington, D.C.) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 687260

Exosomes (Exos) are emerging as noninvasive biomarkers for diagnosis and progression monitoring of gastric cancer (GC). However, the heterogeneity discrimination and ultrasensitive quantification of Exos presents a considerable analytical challenge, thereby impeding severely their clinical application. Herein, we propose an integrated terahertz metasensing platform for the discrimination of Exos in distinct subtypes of GC in a single step-through the simultaneous evaluation of the category and richness level of Exos membrane proteins. Characterized by dual-sided independent sensing capabilities with enhanced sensitivity (169 and 325 GHz per refractive index unit, respectively), the metasensor functionalized with antibodies simultaneously reflects the content of 2 membrane proteins in the terahertz spectral response. Our approach concurrently completes accurate differentiation and precise quantification of GC-subtype Exos by integrating dual-sided sensing information in merely a single assay. The dual-sided sensing design enhances the reliability of detection results. Moreover, combined with the signal amplification of gold nanoparticles, the platform experimentally demonstrates a superior dynamic response to Exos concentrations spanning from 1 × 10
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