METHODS: The Capsid protein (CP) of APV1 was expressed in RESULTS: The MAbs APV1CP-1 and APV1CP-10 were successfully obtained with titers exceeding 1:102,400. Colloidal gold particles used in the assay had an approximate diameter of 30-40 nm, and exhibited a surface plasmon resonance peak around 530 nm. The CGICS allowed for the detection of APV1 by applying infected sap to the test strip, with results visible within 5-10 min. The test showed no cross-reactivity with other viruses tested, and the visual detection limit for APV1 was established at a 100-fold dilutions of APV1-infected leaf samples. CONCLUSION: The monoclonal antibody-based colloidal gold immunochromatographic strip developed in this study demonstrates significant convenience, rapidity, and reliability for APV1 detection. These advancements are anticipated to facilitate rapid diagnosis and surveillance of APV1 in field settings.