Bone defects resulting from trauma, tumor resection, non-union of fractures, and infections present enormous challenges in treatment. Although three-dimensional (3D) bioprinting plays an important role in repairing bone tissues, the lack of mechanical properties and osteoinductive ability of the bioinks remains a barrier for the application of the technology. In this study, we used advanced 3D bioprinting technology to create a novel piezoelectric hydrogel scaffold (Gel/PBT@BMSCs) which consisted of bone marrow-derived mesenchymal stem cells (BMSCs), gelatin methacryloyl (GelMA), and polyethylene glycol (PEG)-modified barium titanate (BT) nanoparticles. The piezoelectric hydrogel scaffold provided a stable 3D microenvironment for cell growth and adhesion, enhancing cell viability and osteogenic activity when subjected to low-intensity pulsed ultrasound (LIPUS) stimulation. Furthermore,