AIMS: 4D flow magnetic resonance imaging (MRI) has enabled evaluation of intracardiac flow dynamics by particle tracing for visualizing and quantifying complex flow patterns. The aim of this study was to assess the impact of respiratory motion compensation on 4D flow MRI-based left ventricular four-component particle tracing, valve tracking, and haemodynamics. METHODS AND RESULTS: In this prospective cohort study, 4D flow MRI with and without respiratory motion compensation was performed in 15 healthy volunteers. Intracardiac particle tracing considered four components: direct flow, delayed ejection flow (DEF), retained inflow (RI), and residual volume. Data quality was assessed by comparing DEF and RI components. Particle tracing, valve tracking, kinetic energy (KE), and vorticity were compared between scan methods. Paired sample CONCLUSION: Respiratory motion compensation can improve accuracy of intracardiac particle tracing based on 4D flow MRI by decreasing mismatch to retrospective valve tracking. For intracardiac particle tracing, respiratory motion compensation is advised. Robust data quality assessment for particle tracing-based analyses is equally crucial.