Inhibition of HDAC4 in granulosa cells improved co-cultured oocyte maturation in vitro independent of LH in porcine.

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Tác giả: Yibing Bao, Hengxing Li, Lin Lin, Chao Wang, Tengteng Wang, Guoliang Xia, Zhenzi Zuo

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: England : Development (Cambridge, England) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 687641

In domestic animals, the mechanisms by which the luteinizing hormone (LH) surge induces oocyte meiosis resumption and maturation through follicular somatic cells remain unclear. Given the pivotal roles of histone deacetylases (HDACs) in regulating gametogenesis, this study investigated the roles of HDACs in follicular granulosa cells (GCs) in mediating LH action during oocyte maturation in pigs. The results showed that histone deacetylase 4 (HDAC4) levels in cultured GCs increased in a time-dependent manner with follicle-stimulating hormone (FSH) stimulation but significantly decreased with LH treatment. The LH-induced reduction of HDAC4 was mediated by the accumulation of extracellular signal-regulated kinase 1/2 (ERK1/2), which subsequently increased H3K18 acetylation and promoted the recruitment of SMAD family member 3 (SMAD3) to the promoter of the EGF-like growth factor amphiregulin (Areg). Notably, specific inhibition of HDAC4 promoted oocyte maturation independently of LH in vitro, and the developmental potential of these matured oocytes was comparable to those induced by LH in vitro. In conclusion, HDAC4 in follicular somatic cells serves as a gonadotrophin-responsive epigenetic modification factor that negatively regulates oocyte meiosis resumption in pigs.
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