Identification of Amino Acid Residues Critical for the Interaction of Fibrin with N-Cadherin.

• Tác giả: Leonid Medved, David A Nyenhuis, Dudley K Strickland, Nico Tjandra, Sergiy Yakovlev
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Biochemistry , 2025
• Bộ sưu tập: NCBI
• ID: 689169

Tóm tắt

We recently identified N-cadherin as a novel receptor for fibrin and localized complementary binding sites within the fibrin βN-domains and the third and fifth extracellular domains (EC3 and EC5) of N-cadherin. We also hypothesized that the His16 and Arg17 residues of the βN-domains and the (Asp/Glu)-X-(Asp/Glu) motifs present in the EC3 and EC5 domains may play roles in the interaction between fibrin and N-cadherin. The primary objectives of this study were to test these hypotheses and to further clarify the structural basis for this interaction. To test our hypotheses, we first mutated His16 and Arg17 in the recombinant (β15-66)