The OsMAPK6-OsWRKY72 module positively regulates rice leaf angle through brassinosteroid signals.

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Tác giả: Qiuhua Cai, Liping Chen, Lili Cui, Yi Huang, Minrong Jiang, Ling Lian, Fuxiang Wang, Yanjia Xiao, Hongguang Xie, Huaan Xie, Jianfu Zhang, Ling Zhang, Yongchao Zhao, Yongsheng Zhu

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: China : Plant communications , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 689218

Leaf angle is a major agronomic trait that determines plant architecture, which directly affects rice planting density, photosynthetic efficiency, and yield. The plant phytohormones brassinosteroids (BRs) and the MAPK signaling cascade are known to play crucial roles in regulating leaf angle, but the underlying molecular mechanisms are not fully understood. Here, we report a rice WRKY family transcription factor gene, OsWRKY72, which positively regulates leaf angle by affecting lamina joint development and BR signaling. Phenotypic analysis showed that oswrky72 mutants have smaller leaf angles and exhibit insensitivity to exogenous BRs, whereas OsWRKY72 overexpression lines show enlarged leaf angles and are hypersensitive to exogenous BRs. Histological sections revealed that the change in leaf inclination is due to asymmetric cell proliferation and growth at the lamina joint. Further investigation showed that OsWRKY72 binds directly to the promoter region of BR receptor kinase (OsBRI1), a key gene in the BR signaling pathway, and activates its expression to positively regulate rice BR signaling. In addition, we discovered that OsWRKY72 interacts with and is phosphorylated by OsMAPK6, and this phosphorylation event can enhance OsWRKY72 activity in promoting OsBRI1 expression. Genetic evidence confirmed that OsMAPK6, OsWRKY72, and OsBRI1 function in a common pathway to regulate leaf angle. Collectively, our findings clarify the critical role of the OsWRKY72 transcription factor in regulating rice leaf angle. These results provide valuable insights into the molecular regulatory networks that govern plant architecture in rice.
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