This study compared the effects of 90° versus 40° hip flexion in the leg extension exercise on quadriceps femoris muscle hypertrophy. Twenty-two untrained men completed a ten-week intervention comprising two resistance training sessions per week. A within-participant design was used, with the lower limb side randomly allocated to the 40 or 90° condition. Muscle thickness of distal and proximal rectus femoris and vastus lateralis was quantified via ultrasound. Data were analysed within a Bayesian framework including univariate and multivariate mixed effect models with random effects to account for the within participant design. Differences between conditions were estimated as average treatment effects (ATE) and inferences were made based on posterior distributions and Bayes Factors (BF). Results indicated a greater hypertrophic response in the rectus femoris for the 40° condition, with "extreme" evidence supporting a hypertrophic response favouring the 40° hip angle for the rectus femoris (BF >
100
p(Distal/ATE & Proximal/ATE >
0) >
0.999), and "strong" evidence supporting no difference in hypertrophic response for the vastus lateralis (BF = 0.07). Therefore, both conditions could be viable options for increasing quadriceps femoris hypertrophy. However, when training for maximizing rectus femoris hypertrophy among untrained men, we suggest training with a reduced hip flexion in the leg extension exercise.