Hormones are effective in regulating plant metabolism and, therefore, are often used in plant cell culture to increase the yield of target products. This study investigated the effects of hormones on the growth and anthocyanin biosynthesis of suspension-cultured red Cyclocarya paliurus cells. Additionally, the mechanism by which gibberellin induces anthocyanin biosynthesis was explored through multi-omics integrated analysis and the assay of the dynamic changes in signaling molecule concentration. The results showed that the total anthocyanin content and yield of suspension-cultured cells, when induced by 1.0 mg L⁻¹ Gibberellin A