BACKGROUND: In emergency warning nursing, the pre-alert system significantly influences the biochemical markers and clinical outcomes of patients with Acute Stress Disorder. Therefore, this study applies hierarchical analysis to explore the impact of early warning nursing on crucial indicators such as C-reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and assess their clinical efficacy. METHODS: The study selected patients with acute stress disorder who were hospitalized in Southwest Hospital of Chongqing from December 2021 to December 2022, and collected data from 250 patients. Through PSM score matching, 170 patients were finally scored and grouped, 85 patients in each group, which were divided into routine group and stratified analysis group. The changes in serum inflammatory markers, psychological resilience, and post-traumatic growth were compared between the two experimental groups on day 1 of admission and after 14 days of intervention. RESULT: After one day of admission, there was no significant difference in the serum factor levels, psychological resilience, and post-traumatic growth among the participants (p >
0.05). However, after 14 days of intervention, patients in the hierarchical analysis group showed better outcomes in serum inflammatory markers such as C-reactive protein, procalcitonin, tumor necrosis factor-alpha, and interleukin-6 compared to the conventional group (p <
0.05). The hierarchical analysis group had higher psychological resilience scores regarding strength, optimism, and resilience compared to the conventional group (p <
0.05). Furthermore, the hierarchical analysis group showed higher post-traumatic growth scores regarding mental changes, personal strength, appreciation of life, interpersonal relationship, and new possibilities relative to the conventional group (p <
0.05). CONCLUSION: Analytic Hierarchy Process (AHP)-based emergency warning nursing can help improve the serum inflammatory factor levels, strengthen psychological resilience, and enhance post-traumatic growth levels in patients with Acute Stress Disorder.