OBJECTIVE: Hotel workers are at risk for burnout. We tested the association between effort-reward imbalance (ERI) and burnout. METHODS: Participants were recruited through social media and completed an online survey. ERI and burnout were analyzed using an adjusted multivariable regression model. RESULTS: Two hundred twenty-four hotel workers were surveyed. Eighty-three percent were female, 38% were guest room attendants, and 19% were in leadership. Most were White (62%), nonunionized (78%), and worked full-time (76%). The mean age was 39.4 (SD = 13). The mean burnout score was 41.8 (SD = 6.6, range of 20.0-59.0). 50.5% of participants reported high ERI, especially among leadership, food workers, and guest room attendants. CONCLUSIONS: High ERI was associated with higher burnout. Older age was associated with reduced burnout. Hotel workers experience high levels of stress. Targeted interventions are necessary to foster coping and mitigate risk factors.