Identification of cold tumor induction-related markers in pancreatic cancer and the clinical implication of PCDH7.

• Tác giả: Toshiya Abe, Noboru Ideno, Naoki Ikenaga, Chika Iwamoto, Akihiro Kubo, Yuki Mochida, Masafumi Nakamura, Kohei Nakata, Yoshinao Oda, Kenoki Ohuchida, Koki Oyama, Tomohiko Shinkawa, Nobuhiro Torata, Chikanori Tsutsumi, Yutaka Yamada, Bo Zhang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 624.3 Girder and related kinds of bridges
• Thông tin xuất bản: Germany : Journal of cancer research and clinical oncology , 2025
• Bộ sưu tập: NCBI
• ID: 690117

Tóm tắt

 PURPOSE: Pancreatic ductal adenocarcinoma (PDAC) is considered a "cold" tumor because the tumor immune microenvironment (TIME) exhibits poor intratumoral T-cell infiltration. This study aimed to identify the marker genes associated with induction of cold TIME in PDAC cells. METHODS: We orthotopically transplanted 10 primary cultures of PDAC derived from KrasG12D/+
  Trp53R172H/+
  Pdx-1-Cre (KPC) mice into immunocompetent mice and evaluated TIME by immunohistochemistry (IHC) staining of CD8. We divided primary cultures into two groups: cold TIME group with low CD8 RESULTS: We identified six genes specific in PDAC cells to the cold TIME group by RNA sequencing
  these were defined as "cold tumor induction-related genes". Human PDAC scRNA-seq data revealed that cold tumor induction-related genes were significantly and negatively correlated with the number of CD8 CONCLUSION: PCDH7 is a prognostic marker associated with CD8