Zeolitic nanosheets possess great potential in catalysis due to their enhanced transport property and accessibility toward bulky molecules compared to conventional micron- meter scale crystals. However, the generation of Beta zeolite nanosheets, which are crucial for industrial catalysis, is still challenging for its intergrowth nature. In this work, aluminosilicate Beta nanosheets of ca. 16 nm thick with house-of-cards architecture are generated using a special polycationic organic structure directing agent (OSDA), [-N