A steam-mediated isothermal amplification and flocculation-based detection platform for electricity-free point of care diagnostics.

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Tác giả: Kissan D Achary, Satheesh Natarajan, Aashish Priye

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: England : The Analyst , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 691631

Approximately 9% of the global population lacks access to reliable electricity, and the absence of affordable, electricity-free diagnostic tools hinders early detection of infectious diseases, exacerbating public health burdens in resource-limited settings. We introduce SteamFloc-LAMP - an electricity-free molecular diagnostic platform engineered for instrument-free detection of pathogenic nucleic acid targets. The platform leverages steam-mediated heating from boiling water to sustain the isothermal conditions required for Loop-Mediated Isothermal Amplification (LAMP). Thermal characterization of the steam-mediated heating system identified parameters that enable the consistent maintenance of optimal temperatures for LAMP reactions with minimal fluctuations. Visual end-point detection was achieved through a bridging flocculation mechanism, which exploits the interaction between LAMP amplicons, spermine, and charcoal particles, leading to visible aggregation in positive samples, thus enabling naked-eye detection without the need for specialized equipment or expensive reagents like fluorophores or colorimetric dyes. The SteamFloc-LAMP assay targeted the
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