Stealth precision polymer nanofibers show great promise as therapeutic delivery systems. However, existing systems are largely limited to poly(ethylene glycol) (PEG) and suffer from challenging functionalization, hampering their translation. This work develops a modular, easily functionalizable platform for biocompatible stealth nanofibers based on a combination of ring-opening polymerisation (ROP), photoinduced electron/energy transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerisation, and crystallization-driven self-assembly (CDSA). Low length-dispersity poly(fluorenetrimethylenecarbonate)-b-poly(N-(2-hydroxypropyl) methacrylamide) (PFTMC-b-PHPMA) nanofibers may be produced in a single-step via CDSA, with a length that is dependent on the PHPMA DP