This study evaluated the antimicrobial efficacy of electrolyzed water (EW) and chlorine-based disinfectant (CLD) over time, focusing on the impact of pH, free chlorine (FCL), and oxidation-reduction potential (ORP). EW and CLD are commonly used for wound care and surgical instrument disinfection, but their chemical instability limits their use. The study was conducted in the Microbiology Laboratory of the University of Guanajuato, using