Lysosome abnormality closely relates to a variety of diseases
thus, its long-time tracking could benefit accurate disease diagnosis. Current long-time lysosome imaging probes are not biocompatible enough, while compatible peptide-based probes are easily degraded by the abundant proteinases in lysosome. Herein, we rationally design a d-amino acid-containing peptide Cys(StBu)-d-Glu-Lys(coumarin)-d-Glu-CBT (Cou-D/L-CBT) which is subjected to intracellular GSH-initiated CBT-Cys click reaction and assembles into nanotubes in acidic lysosome.