Small extracellular vesicles (EVs) are a subtype of nano-sized extracellular vesicles that mediate intercellular communication. EVs can be found in different body fluids, including milk. Monocytes internalize porcine milk EVs and modulate immune functions in vitro by decreasing their phagocytosis and chemotaxis while increasing their oxidative burst. This study aimed to assess the impact of porcine milk EVs on the porcine peripheral blood mononuclear cells (PBMC) proteome. Porcine PBMC were incubated with porcine milk EVs or medium as a control. Extracted proteins were then analyzed using nano-LC-MS/MS. A total of 1584 proteins were identified. The supervised multivariate statistical analysis, sparse variant partial least squares - discriminant analysis (sPLS-DA) for paired data identified discriminant proteins (DP) that contributed to a clear separation between the porcine milk EVs treated cells and control groups. A total of 384 DP from both components were selected. Gene Ontology (GO) enrichment analysis with ProteINSIDE provided the evidence that the DP with a higher abundance in porcine milk EVs, like TLR2, APOE, CD36, MFGE8, were mainly involved in innate immunity and the process of EVs uptake processes. These results provide a proteomics background to the immunomodulatory activity of porcine milk EVs and to the potential mechanisms used by immune cells to internalize them.