Carbon monoxide inhibits human bronchial epithelial CCL5 and IL-6 secretion induced by SARS-CoV-2 spike RBD protein.

• Tác giả: Xiao-Min Fang, Xing-Jian Liu, Rui-Gang Zhang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 332.31 Agricultural institutions
• Thông tin xuất bản: United States : Experimental cell research , 2025
• Bộ sưu tập: NCBI
• ID: 693401

Tóm tắt

Carbon monoxide (CO) is a novel anti-inflammatory molecule, but the effects of CO on SARS-CoV-2 spike RBD (S-RBD)-induced human bronchial epithelial cytokines release remains unclear. CO was delivered using CO-releasing molecule 3 (CORM-3). The effects of S-RBD, ATPγS and CO on cytokines secretion were determined by enzyme-linked immunosorbent assay (ELISA) in 16HBE14o-human bronchial epithelial cell line. The inhibitory effect of CO on S-RBD-induced ERK phosphorylation was assessed by Western blot analysis. The regulatory effect of CO on extracellular nucleotide-induced ion transport was quantified by short-circuit current (I