One of the key challenges in using natural extracts for water treatment is their biodegradability and susceptibility to microbial spoilage, which can limit storage and long-term effectiveness. This study investigates the scale inhibition capabilities of an aqueous extract of Salvia rosmarinussp through electrochemical measurements, conductivity tests, and morphological examination. Additionally, two natural substances, Rhamnolipid and Chitosan, were evaluated as bio-preservatives to prevent mold growth and enhance the shelf life of the rosemary extract. The reasons for selecting these specific bio-preservatives include their known antimicrobial properties, antioxidant effects, environmental benefits, and suitability for the intended application. For 24 weeks, we conducted a microbial examination and assessed the anti-scaling performance of the extract in combination with the bio-preservatives. The results demonstrate that rosemary extract significantly inhibits CaCO