INTRODUCTION: Most youth do not meet national nutrition recommendations and overconsume high-calorie, low nutrient-dense foods. Adequate nutritional intake is crucial for growth and development. Nutrition practices play a key role in sports performance and recovery. Nutritional knowledge can affect eating patterns. However, there is limited research on general and sport-specific nutrition knowledge and behaviors of adolescent athletes. PURPOSE: To examine general and sport-specific nutrition knowledge and behaviors of adolescent athletes. METHODS: Adolescent athletes were assessed on nutrition knowledge and general and sport-specific nutrition behaviors, including food group consumption, hydration practices, and sport supplement use. Gender differences in general and sport-specific nutrition behaviors were compared using a Mann-Whitney U test. Odds ratios and logistic regression, controlling for age, ethnicity, and years in sport participation were used to determine differences in correct responses between genders. Significance was set at the RESULTS: One hundred and ninety-four athletes ( CONCLUSION: Adolescent athletes report lower than recommended food group consumption and lack knowledge of general and sport-specific nutrition information. Improvements in these areas can play a significant role in the health and performance of adolescent athletes.