Insects have evolved a diversity of regulatory mechanisms to determine their sex. Understanding the molecular regulation mechanisms of insect sex determination is of great significance in revealing the general law of insect sex determination and providing potential routes for the genetic manipulation of pest species. Although the sex determination cascade and doublesex (dsx) gene functions have been well described in some holometabolous insects, little is known about this cascade in hemimetabolous insects. In this study, we identified the dsx homolog in Gryllus bimaculatus, which belongs to the Orthoptera order and is an important model for developmental and evolutionary biology. We found that Gbdsx has two alternative splicing isoforms (male-specific Gbdsx