In recent years, urgent food safety issues have heightened the demand for rapid detection technologies for foodborne pathogens, especially biosensors featuring simplicity, rapidity, and high sensitivity. Yet despite a booming surge in related published studies, commercializing these biosensors remains a constant challenge and persistent objective for researchers. In this study, a gravity-driven microfluidic chip with tilt-actuated siphon valves was developed, integrating silica magnetic beads based nucleic acid separation and recombinase-aided amplification (RAA) detection of