There is a demand and widespread interest in evaluating microbial community structures and metabolic processes in hydrocarbon environments. The current work aims to detect microbial subgroups (phenotypic subsets) and their metabolic processes, such as substrate specificity and expression of niche-associated genes. In this study, we were able to discriminate different cell types in real time from a complex sample matrix to allow the detection of live, dead, and injured cell populations in jet fuels. We found that the expression of