Third-degree burns are severe thermal injuries that require prolonged recovery periods and are associated with significant sequelae. Photobiomodulation therapy (PBMT) has emerged as a promising approach for treating skin injuries, although parameter selection remains controversial. This study investigated the effects of PBMT using red (wavelength 660 nm) and infrared (wavelength 808 nm) lasers on tissue repair in third-degree burns in rats. Twenty-four male Wistar rats were divided into three groups: control group (CG, laser off), red laser group (RLG, 660 nm), and infrared laser group (IRLG, 808 nm). Treatments were performed three times per week for 21 days, totaling nine applications, with parameters of 3 J energy, 100 mW power, and 5 points distributed around the lesion. Both the RLG and IRLG groups demonstrated faster wound healing and better tissue organization compared to the CG, along with increased collagen deposition. The IRLG group showed superior histological results, indicating greater efficiency in tissue repair. This study demonstrates the effectiveness of PBM with red (660 nm) and infrared (808 nm) lasers in accelerating full-thickness burn healing in rats, with 808 nm showing superior results. The findings highlight PBM's potential in enhancing collagen synthesis and its promise as a therapy for burn recovery.