Mechanical testing of battery components is essential for understanding their mechanical behavior and developing accurate numerical models. Here, we present a protocol for characterizing the mechanical properties of lithium-ion batteries. We describe steps for performing tests on compression, tension, and indentation to evaluate the individual components. Furthermore, we detail procedures for compression testing on stacked structures to assess the mechanical properties of the battery jelly roll, providing key insights into its performance under real-world conditions. For complete details on the use and execution of this protocol, please refer to Wang et al.