The favorable success rate in cancer treatment predominantly depends on precise diagnosis with target-specific drug delivery, which can regulate the patient survival outcome rate. Moreover, proper tracking of the system's pH is very much crucial as most of the therapeutic's action and release rate depend on it. Therefore, this work has been intended to fabricate a folic acid-derived carbon dot (FACD) decorated with chitosan (Cs) in order to form nanospheres (FACD-Cs-Ns) for anticancer doxorubicin hydrochloride (Dox.HCl) drug delivery through imaging in cancer therapeutic treatment. The engineered FACD-Cs-Ns demonstrated a spherical shape with an extensive surface area, rich in carboxyl and hydroxyl groups that play a key role in its pH-responsive characteristics through protonation and deprotonation interactions. Thanks to their impressive fluorescence traits and excellent stability, FACD-Cs-Ns are particularly well suited for imaging-guided cancer therapy. Their remarkable cytocompatibility with normal cells and significant toxicity toward cancer cells, along with pH-responsive properties, render them as ideal candidates for targeted drug delivery to cancer cells. The G2/M and S phases' arrest in the cell cycle analysis study once more validated excellent