BACKGROUND AND AIMS: The influence of dietary fatty acids (FAs) on carotid atherosclerotic plaque components remains unknown. We aimed to assess the association of dietary saturated (SFA), mono-unsaturated (MUFA), and poly-unsaturated FAs (PUFA) with the incidence of carotid plaque components. METHODS: Within the population-based Rotterdam Study, 570 stroke-free participants (mean age: 68 years
47 % women) with subclinical carotid atherosclerosis underwent two serial carotid MRI (mean scan interval: 5.9 years). Carotid calcification, lipid-rich necrotic core (LRNC) and intraplaque hemorrhage (IPH) were evaluated. Intake of dietary FAs was assessed by food-frequency questionnaires around the time of baseline MRI. We used generalized estimating equation to investigate the association between dietary FAs and new onset of carotid plaque components. RESULTS: We found that a higher PUFA intake was associated with a lower incidence of IPH (adjusted odds ratio (OR) per 5 g/d increase: 0.80 (95 % confidence interval (CI): 0.65 to 0.98)). In the substitution models, a modelled higher PUFA intake at the expense of MUFA or SFA was also associated with a lower risk of incident IPH, with adjusted ORs per 5 g/day more PUFA of 0.81 (95 % CI: 0.58 to 1.14) for replacing SFA and 0.60 (95 % CI: 0.37 to 0.97) for replacing MUFA. No associations between SFA or MUFA and the carotid plaque components were found. CONCLUSION: Among persons with subclinical carotid plaque, dietary FA intake is a modifiable risk factor for changes in carotid plaque vulnerability. Diet with more PUFA is associated with a lower risk of IPH in carotid plaque.