The emergence and spread of antimalarial drug resistance pose significant challenges in the fight against malaria. Mutations in dihydrofolate reductase (DHFR) and dihydropteroate synthetase (DHPS) in Plasmodium vivax are associated with sulfadoxine-pyrimethamine (SP) drug resistance. This study assessed SP resistance status in P. vivax isolates collected in Khyber Pakhtunkhwa province, Pakistan, by analyzing mutations in pvdhfr and pvdhps. Both genes were successfully amplified concurrently from 112 Pakistan P. vivax isolates. Sequence analysis of pvdhfr indicated that mutations F57L, S58R, and S117N were present with frequencies of 0.9 %, 31.3 %, and 46.4 %, respectively. The predominant wild-type haplotype F