New methodologies to infer past evolutionary, ecological and biogeographical processes from molecular phylogenies are rapidly being developed. However, these often employ unfamiliar data structures that may pose a barrier to their use. DAISIE (Dynamic Assembly of Islands through Speciation, Immigration and Extinction) is an island biogeography model that can estimate rates of colonisation, speciation and extinction from molecular phylogenetic data across insular assemblages. The method uses an unconventional phylogenetic data structure: instead of considering a single island lineage, it focuses on multiple independent lineages descending from different colonisation events of the island. While analyzing phylogenies from this perspective has plenty of potential, this comes with challenges for the user. Here we describe software DAISIEprep, an R package to aid the extraction of data from one or many phylogenetic trees to generate and visualise data in a format interpretable by macroevolutionary and biogeographical inference models. DAISIEprep includes simple algorithms to extract data on island colonists and account for biogeographical, topological and taxonomic uncertainty. It also allows flexible incorporation of either missing species or entire insular lineages when molecular data are not available. The software enables reproducible and user-friendly data extraction, formatting and visualisation of phylogenetic data from island lineages, and will facilitate addressing questions about island evolution, community ecology and anthropogenic impacts in insular systems. The tools presented here will also be useful for researchers who do not plan to use DAISIE but are interested in how to interpret, visualize and analyze phylogenetic datasets of islands species or island-like environments.