This case-control study aimed to investigate the health risks faced by university professors as a result of using whiteboard markers. The study included 30 professors who used the whiteboard markers for teaching and 20 professors who used other teaching aids. Samples of urine and breathing air were collected from the participants, and then analyzed in the laboratory using gas chromatography (GC) to measure the concentrations of BTEX compounds. The carcinogenic and non-carcinogenic risks of these compounds were assessed using a probabilistic method. The mean concentrations (ug/L) of compounds in the urine differed significantly (p <
0.05) between the case and control groups for benzene (82.59 vs. 65.36), toluene (128.47 vs. 85.65), and ethylbenzene (9.09 vs. 25.16). The mean lifetime cancer risk (LTCR) for benzene (8.27 × 10