Water spinach (Ipomoea aquatica Forsk.) is an important leaf vegetable affected by salt stress, however, little is known about its salt adaption mechanism. Here, we integrated physiomics, ionomics, transcriptomics, and metabolomics to analyze the root adaptation response of two water spinach varieties, BG (salt-tolerant) and MF (salt-sensitive), at 150 mM NaCl. The results showed that compared with MF, BG significantly reduced the content of malondialdehyde (MDA) and H