BACKGROUND: This study was designed to evaluate content validity of the Dimensional Anhedonia Rating Scale (DARS), a patient-reported outcome measure, in adults with anhedonia in the context of major depressive disorder (MDD). To accomplish this, a conceptual model including the symptoms and impacts of anhedonia in the context of MDD was developed and refined through a targeted literature review, clinician interviews (N = 6), and participant interviews (N = 20). RESULTS: Using the final conceptual model, an item mapping exercise was conducted for the DARS, demonstrating that it provided suitable concept coverage in this population. Cognitive debriefing of the DARS with participants demonstrated that it was generally well understood and clear. CONCLUSIONS: Overall, the study established that the DARS demonstrates content validity in adults with anhedonia in the context of MDD. Other measurement properties of the DARS will be evaluated in planned psychometric analyses.