There is an urgent need today for interface management with recognition layers composed of short receptor molecules, with excellent specificity and affinity toward a target molecule, for a wide range of sensing applications. The current work demonstrates a specific detection of a G-type nerve agent, which is based on a nucleophilic substitution reaction between the surface-bound 4-amino-2-((dimethylamino)methyl)phenol (amino-2-DMAMP) receptors and the diethyl chlorophosphate (DCP) simulant. The specificity and affinity of 2-DMAMP toward DCP are demonstrated with